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GSH can be regenerated from GSSG by the enzyme glutathione reductase (GSR): NADPH reduces FAD present in GSR to produce a transient FADH-anion

This was performed to avoid subsequent proliferation-induced hit biases - prominent with loss of mitochondrial genes - when comparing conditions 24,25

If your protocol will take longer than that to use a full vial, reconstitute smaller amounts more frequently

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42 In another study in beagles in a research setting, measures of oxidative stress (malondialdehyde or MDA) were higher in older dogs, along with apparently compensatory increases in both glutathione peroxidase and SOD activities